Plasmid

Part:BBa_K1971000:Design

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-14)


psb1c3-ompt-crm197


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2056
    Illegal suffix found in sequence at 8
    Illegal XbaI site found at 2140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2056
    Illegal SpeI site found at 9
    Illegal PstI site found at 23
    Illegal NotI site found at 16
    Illegal NotI site found at 2062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2056
    Illegal XhoI site found at 1040
    Illegal XhoI site found at 1932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2056
    Illegal suffix found in sequence at 9
    Illegal XbaI site found at 2140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2056
    Illegal XbaI site found at 2071
    Illegal XbaI site found at 2140
    Illegal SpeI site found at 9
    Illegal PstI site found at 23
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal SapI.rc site found at 2369


Design Notes

the constuction of PET28a-ompt-CRM197 is tough


Source

all from artificial sequence.

References